US10130701.pdf

(4846 KB) Pobierz
US010130701B2
(12) United States Patent
Bickerton et al.
(54 ) CORONAVIRUS
(71) Applicant: THE PIRBRIGHT INSTITUTE ,
Pirbright, Woking (GB )
( 72 ) Inventors : Erica Bickerton , Woking (GB ); Sarah
Keep , Woking (GB ); Paul Britton ,
Woking (GB )
(73 ) Assignee : THE PIRBRIGHT INSTITUTE ,
Woking, Pirbright (GB )
(45) Date of Patent:
( 10) Patent No.: US 10 ,130,701 B2
Nov . 20 , 2018
OTHER PUBLICATIONS
Sperry Journal of Virology, 2005, vol. 79 , No . 6 , pp . 3391 - 3400 .*
Altschul et al., Basic local alignment search tool. J. Mol. Biol. 215 :
403 - 10 ( 1990 ) .
Ammayapppan et al., Identification of sequence changes respon
sible for the attenuation of avian infectious bronchitis virus strain
Arkansas DPI, Arch . Virol., 154 ( 3 ) :495 - 9 (2009 ).
Anonymous: “ EM _ STD :KF377577” , Oct. 30 , 2013 .
( * ) Notice :
patent is extended or adjusted under 35
U . S . C . 154 ( b ) by 0 days .
Subject to any disclaimer, the term of this
Armesto et al., A recombinant avian infectious bronchitis virus
expressing a heterologous spike gene belonging to the 4 /91 serotype ,
PLoS One, 6 ( 8 ):e24352 (2011) .
Armesto et al., The replicase gene of avian coronavirus infectious
bronchitis virus is a determinant of pathogenicity, PLoS One,
4 ( 10 ):e7384 (2009).
Armesto et al., Transient dominant selection for the modification
( 21) Appl. No .:
( 22 ) PCT Filed :
15 /328 ,179
Jul. 23, 2015
(86 ) PCT No.:
$ 371 (c )( 1),
( 2 ) Date :
PCT/GB2015/052124
Jan . 23 , 2017
Methods Mol. Biol., 454:255 - 73 ( 2008) .
Ausubel et al., Short Protocols in Molecular Biology , 4th edition ,
Chapter 18 ( 1999 ) .
Britton et al., Generation of a recombinant avian coronavirus
and generation of recombinant infectious bronchitis coronaviruses,
Virol. Methods, 123 ( 2 ): 203 - 11 (2005).
bronchitis virus for vaccine development, Bioeng . Bugs., 3 (2 ): 114 -9
infectious bronchitis virus using transient dominant selection , J.
( 2012 ) .
Casais et al., Recombinant avian infectiousbronchitis virus express
Britton et al., Modification of the avian coronavirus infectious
(87) PCT Pub . No.: WO2016 /012793
PCT Pub . Date : Jan . 28 , 2016
Prior Publication Data
(65 )
US 2017 /0216427 A1 Aug . 3 , 2017
Foreign Application Priority Data
(30 )
Devereux et al., A comprehensive set of sequence analysis programms
for the VAX . Nucl. Acids Res. 12 : 387 - 95 (1984 ).
Cavanagh et al.,Manipulation of the infectious bronchitis coronavirus
ing a heterologous spike gene demonstrates that the spike protein is
a determinant of cell tropism , J. Virol., 77 (16 ):9084 -9 ( 2003 ).
Casais et al., Reverse genetics system for the avian coronavirus
infectious bronchitis virus, J. Virol., 75 (24 ):12359 -69 (2001).
genome for vaccine development and analysis of the accessory
Jul. 23, 2014 (GB ) ............................ 1413020.7
(51) Int . CI.
A61K 39 /215
C12N 7700
( 2006 .01)
( 2006 .01)
C12N 9 /12
A61K 39 / 00
(52)
U .S . CI.
( 2006 .01)
( 2006 .01)
International Search Report and Written Opinion , International
Application No. PCT/GB2015 /052124 , dated Oct. 9 , 2015 .
Larkin et al., Clustal W and Clustal X version 2 .0 , Bioinformatics,
23 ( 21 ): 2947-8 ( 2007).
Menachery et al., Attenuation and restoration of severe acute
respiratory syndrome coronavirus mutant lacking 2 ' -0
methyltransferase activity , J. Virol., 88 (8 ): 4251 -64 (2014 ).
protein and nucleotide sequences , FEMSMicrobiol. Lett ., 174 ( 2 ): 247
50 ( 1999) .
proteins , Vaccine , 25 ( 30 ): 5558 -62 ( 2007 ) .
International Preliminary Report on Patentability, International Appli
cation No. PCT/GB2015 /052124 , dated Jan . 24 , 2017.
CPC .............. A61K 39/215 (2013 .01) ; C12N 7700
(2013 .01 ) ; C12N 9 / 127 ( 2013 .01 ) ; C12Y
Tatusova et al., BLAST 2 Sequences, a new tool for comparing
207/07048 (2013 . 01 ); A61K 2039 / 5254
( 2013 .01) ; A61K 2039/ 54 ( 2013 .01 ); C12N
2770 / 20021 ( 2013 .01) ; C12N 2770 / 20022
(Continued )
( 2013.01); CI2N 2770 /20034 ( 2013 . 01) ; C12N
2770/ 20051 ( 2013.01); C12N 2770 /20062
( 2013.01)
(58 ) Field of Classification Search
CPC . .. . .. . .. .. ..
Primary Examiner — Bao Q Li
(74 ) Attorney, Agent, or Firm — Marshall, Gerstein &
Borun LLP
See application file for complete search history .
References Cited
(56 )
............. A61K 39 /215
(57)
ABSTRACT
U .S. PATENT DOCUMENTS
7,452, 542 B2 * 11/2008 Denison . .............
CO7K 14 /005
424 / 221. 1
The present invention provides a live , attenuated coronavi
rus comprising a variant replicase gene encoding polypro
teins comprising a mutation in one or more of non - structural
FOREIGN PATENT DOCUMENTS
WO
protein (s) ( nsp )- 10 , nsp - 14 , nsp - 15 or nsp - 16 . The corona
virus may be used as a vaccine for treating and/or preventing
a disease , such as infectious bronchitis, in a subject.
WO - 2004 /092360 A2
WO - 2005 / 049814 A2
10 /2004
6 / 2005
WO
WO
WO
WO - 2007/078203 A1
WO - 2011 /004146 Al
7 /2007
1 / 2011
25 Claims, 15 Drawing Sheets
Specification includes a Sequence Listing.
US 10 ,
Page
,
2
701 B2
130
( 56 )
References Cited
OTHER PUBLICATIONS
Wang et al., Attenuation of porcine reproductive and respiratory
syndrome virus strain MN184 using chimeric construction with
vaccine sequence , Virology, 371(2 ):418 -29 (2008 ).
Wei et al., Development and characterization of a recombinant
infectious bronchitis virus expressing the ectodomain region of S1
gene of H120 strain , Appl. Microbiol. Biotechnol., 98 (4 ): 1727 - 35
( 2014 ).
* cited by examiner
atent
Nov . 20 , 2018
Sheet 1 of 15
US 10 , 130, 701 B2
FIGURE 1
M41 growth curve
AGEN
WWLLLLLLLLLLLW
KANAKAN
V UTO
CONTACONSANT
p)
log10
Titremluf(i
LLL
*
1141 R - 6
mon M41 R - 12
M41 EP4
+ + + + + + + + +
+ + + + + + + + + + + + + + + +
20
40
Time -hours
atent
Nov . 20 , 2018
Sheet 2 of 15
US 10 , 130, 701 B2
FIGURE 2
1.80
1 .60
rent
for
ber nea
+
Snicking
mock
Beau - R
V M41 - R 6
7
27
,
Nofebmusperkcinbirdrs
*
!
7
vorbeitn
minute
e e e
Ader
present
forward
1.40
0 .60
0 .40
M41- R 12
WM41- CK EP4
/
17
.
42
0.20
0 .00
'
'
' ' ' '' ' ' ' ' ' '' ' ' ' ' ' ' ''
?
eeeee
/
711
#
*
Day 3
S
SSSSSS
S
\ \
Day 4
\ \ \
\
\ \\
Day 5
Day 6
Day 7
SSSSSS244444
ARFAU
P
AP
+
2
12
.
1
*
*
m
m
Wheezing
mock
Beau - R
FIRE
M41-R 6
W M41- R 12
W M41- CK EP4
Day 3
Day 4
Day 5
Day 6
Day 7
atent
Nov . 20 , 2018
Sheet 3 of 15
US 10 , 130 ,701 B2
FIGURE 3
M
TKKAASSA k ha AAAAtt
A
AA % 7 , 1 M M M M M M M M M M M M M
M M M
MAKKAANAK
KAN
MAN
AT, T M M MM
AAAAAAAA
NAMAKKA A
MANT A KA, T , A , P , N , M ,M , N , M , N , M , N , M , N , M ,N , N , M , S , N , M , K ,NANANANAWWWWANAU N N N N N N N N N N , M ,P , N , M , N , M , N , M , M , M ,M , M , M , N , M , N , M ,NN AAAAAAAAKKKAANNNNN
jojuauss?ssy !Áell APV! A
.
.
.
0?1 0?101?7
/
.
4144
1*4775783131#79x.18y444318y4111717
3 87
Pcaeirtlvnargye
?012
11
????
(
????0111170p??171?18 27?5
WWW
ility
W
W mock
1/472
N neag- y
I9WN- # 9
M41- R 12
M41- CK EP4
| hed t
ghea
Lkea

Plik z chomika:

Inne pliki z tego folderu:

Inne foldery tego chomika:

Zgłoś jeśli naruszono regulamin