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the Axon CNS Guide

A GuIde to eleCtropHySIoloGy & BIopHySICS lABorAtory teCHNIqueS

The Axon CNS Guide to

Electrophysiology & Biophysics

Laboratory Techniques

Part Number 2500-102 Rev B

200

Printed in U.S.A.

The Axon CNS Guide



reproduced, stored in retrieval system, or transmitted, in any form or by any means, electronic,

mechanical, photocopying, microfilming, recording or otherwise, in whole or in part for any

purpose whatever, without written permission from the publisher. For information address

Molecular Devices Corporation,

3280 Whipple Road, Union City, CA 94587-1217.

DISCLAIMER

All product names mentioned in this guide are registered trademarks of their respective

manufacturers. Any mention of specific products should not be construed as an endorsement by

Molecular Devices Corporation.

PREFACE

Molecular Devices Corporation is pleased to present you with The Axon CNS Guide (2

ed.), a labora-

tory guide to electrophysiology and biophysics. The purpose of this guide is to serve as an information

and data resource for electrophysiologists. It covers a broad scope of topics ranging from the biological

basis of bioelectricity and a description of the basic experimental setup to a discussion of mechanisms of

noise and data analysis.

The Axon CNS Guide second edition is a tool benefitting both the novice and the expert electrophysiolo-

gist. Newcomers to electrophysiology will gain an appreciation of the intricacies of electrophysiological

measurements and the requirements for setting up a complete recording and analysis system. For experi-

enced electrophysiologists, we include in-depth discussions of selected topics, such as advanced methods

in electrophysiology and noise.

However, as time passes, so has the field of electrophysiology evolved, as well as the depth and com-

plexity of instruments and software. Since the first edition of this guide was originally published, much

of the computer and programming examples have become dated over the years. Nevertheless, we believe

that this reprint remains a valuable contribution to our customers and to the field of electrophysiology

and biophysics.

This guide was the product of a collaborative effort of many researchers in the field of electrophysiology

and of Molecular Devices/Axon Instruments staff. We are deeply grateful to these individuals for sharing

their knowledge and devoting significant time and effort to this endeavor.

David Yamane

Director, Electrophysiology Marketing

Molecular Devices

October 2006

AXON CNS GUIDE

INTRODUCTION

Molecular Devices designs and manufactures instrumentation, reagents and software for a wide

variety of life sciences disciplines and drug discovery applications. As part of a world-class array of

products, the well-known Axon Instruments line of conventional electrophysiology hardware and

software products now comprise the Molecular Devices line of Axon CNS products.

In parallel, Molecular Devices has also developed higher throughput automated electrophysiology

systems that have now become accepted components of the drug discovery process. Ion channel

research now spans a broad spectrum of institutions, from academic institutions to biotech startups

and pharmaceutical corporations.

In recognition of the the continuing excitement in ion channel research, as evidenced by the influx

of molecular biologists, biochemists, and pharmacologists into this field, Molecular Devices is

proud to support your pursuit of electrophysiological and biophysical research with this laboratory

techniques workbook, The Axon CNS Guide.

Acknowledgment to Molecular Devices Consultants and Customers

Molecular Devices employs a talented team of engineers and scientists dedicated to designing

instruments and software incorporating the most advanced technology and the highest quality.

Nevertheless, it would not be possible for us to enhance our products without close

collaborations with members of the scientific community. These collaborations take many

forms.

Some scientists assist Molecular Devices on a regular basis, sharing their insights on current

needs and future directions of scientific research. Others assist us by virtue of a direct

collaboration in the design of certain products. Many scientists help us by reviewing our

instrument designs and the development versions of various software products. We are grateful

to these scientists for their assistance. We also receive a significant number of excellent

suggestions from the customers we meet at scientific conferences. To all of you who have

visited us at our booths and shared your thoughts, we extend our sincere thanks. Another source

of feedback for us is the information that we receive from the conveners of the many excellent

summer courses and workshops that we support with equipment loans. Our gratitude is extended

to them for the written assessments they often send us outlining the strengths and weaknesses of

Molecular Devices’ Axon CNS products.

Axon_Guide.pdf

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